Infographic: How Pure is Your Oligo?

Instruments and Solutions for Oligonucleotide Purity Analysis

Oligonucleotide therapeutics are revolutionizing treatments for various diseases, increasing the demand for precision in oligo synthesis and purity analysis. Well-structured workflows for oligonucleotide purity analysis are essential to meet the rigorous quality standards required for successful downstream applications and eventual manufacturing scale-up.

Challenges in Oligonucleotide Synthesis

Producing high-quality oligos involves addressing specific challenges, such as achieving consistent purity and minimizing sequence-related impurities. The presence of failed sequences or other impurities can compromise the efficacy and stability of synthesized oligonucleotides, affecting their performance in research and therapeutic applications.

• Purity analysis is central to optimizing yields and ensuring that synthesized oligonucleotides meet required quality standards.
• Target plus impurity analysis provides high-resolution accurate mass characterization of target oligos and detects low-abundance impurities, which are critical for monitoring quality and stability over time.

Instruments and Technologies for Streamlined Workflows

Modern advancements in oligo synthesis instruments support each step of the workflow—from sample preparation and separation to detection and final analysis. Precision instruments optimize processes such as:

1. Sample Preparation: Automated preparation tools help streamline the initial stages of oligo synthesis, reducing variability and minimizing human error.
2. Separation: Advanced chromatography systems enhance the separation of oligos from impurities, allowing for highly selective and reproducible results.
3. Detection: High-sensitivity detection tools, such as mass spectrometry, offer precise impurity identification, improving the confidence in purity assessments.
4. Oligo Analysis: Comprehensive analysis software integrates data across each step, providing insights into oligo quality, stability, and efficacy.

Agilent offers a full suite of instruments designed for every stage of the oligonucleotide synthesis and purity analysis workflow. Whether conducting routine purity checks or performing detailed target plus impurity analyses, Agilent’s technologies provide accurate, reliable results to meet the demands of oligonucleotide research and manufacturing.

Download the infographic to learn more.