The Tecan SpectraFluor is a high-performance microplate reader renowned for its precision. The SpectraFluor offers a broad fluorescence detection range, allowing precise measurement of fluorescence signals across a wide spectrum of wavelengths, from ultraviolet to near-infrared, catering to diverse assay requirements and fluorophore characteristics. With exceptional sensitivity and a wide dynamic range, the instrument enables accurate quantification of fluorescence signals, even at low concentrations and high signal intensities, ensuring reliable results for a variety of fluorescence-based assays. Equipped with versatile assay formats, including endpoint, kinetic, and spectral scanning modes, the SpectraFluor accommodates a wide range of fluorescence-based assays, such as nucleic acid quantification, protein detection, enzyme activity assays, and cellular imaging. Featuring automation capabilities for plate handling and data analysis, the instrument enhances workflow efficiency, enabling high-throughput screening and streamlined data acquisition. Designed with an intuitive interface and software, the SpectraFluor offers easy instrument control, data visualization, and analysis, facilitating user-friendly operation and enhancing productivity. The Tecan SpectraFluor is utilized in various research and clinical applications, including drug discovery, molecular biology, immunology, and cell-based assays, where precise and quantifiable fluorescence detection is essential for accurate data analysis and interpretation. In summary, the Tecan SpectraFluor combines a broad fluorescence detection range, high sensitivity, versatile assay formats, automation capabilities, and a user-friendly interface to provide researchers with a reliable and efficient tool for quantifiable fluorescence measurements in microplate assays.