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Learn the Key to Optimizing Your Western Blot

The simplicity and efficiency of the dot blot make it an essential tool for your lab

Did you know that in some cases, using fewer antibodies can result in more signal? Or, conversely, that using more antibody can result in less signal?

A dot blot is a simplified version of a western blot that is performed to optimize antibody concentrations and help ensure accurate and reliable western blot results. Dot blotting helps reduce the time and complexity of the experimental workflow that would otherwise be needed to assess antigen–antibody interactions for further western blot experiments.

This application note explores key aspects of dot blotting including:

The materials needed and steps involved in a successful dot blot procedure
Considerations for assessing the right detection reagents for your application
Guidance on the various types of chemiluminescent substrates available to match your needs

Also included are links to additional resources such as a guide to blocking buffers, an antibody selection criteria tool, an antibody dilution calculator, and other useful resources.

Download the application note to learn more.

DOWNLOAD THIS APPLICATION NOTE

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